by XMRV Global Advocacy on Monday, June 27, 2011:
The following was posted on mecfsforum.com by Gerwyn:
The paper below, from Ramon Mendoza, Andrew E. Vaughan, and A. Dusty Miller, made the following claim:
"The left half of XMRV is present in an endogenous retrovirus of NIH/3T3 Swiss mouse cells"
Here we used XMRV-specific PCR to search for a more closely related source of XMRV in mice. While we could not find a complete copy, we did find a 3,600 bp region of XMRV in an endogenous retrovirus present in NIH/3T3 cells. These results show that XMRV has clear ancestors in mice, and highlight another possible source of contamination in PCR assays for XMRV.
http://jvi.asm.org/cgi/content/abstract/JVI.05137-11v1
However, the following paper demonstrates that NIH/3T3 cells cannot be infected by XMRV.
"Common Inbred Strains of the Laboratory Mouse That Are Susceptible to Infection by Mouse Xenotropic Gammaretroviruses and the Human-Derived Retrovirus XMRV"
In contrast, the XPR1 receptor gene originally cloned from NIH 3T3 cells is not permissive for X-MLV entry (2, 42, 50).
http://jvi.asm.org/cgi/reprint/84/24/12841.pdf
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