Viruses 2011, 3(12), 2442-2461:
The Human Lung Adenocarcinoma Cell Line EKVX Produces an Infectious Xenotropic Murine Leukemia Virus
Laboratory of Cancer Prevention, National Cancer Institute-Frederick, Frederick, MD 21702, USA
* Author to whom correspondence should be addressed.
Received: 26 October 2011; in revised form: 22 November 2011 / Accepted: 9 December 2011 / Published: 19 December 2011
Abstract: The cell lines of the NCI-60 panel represent different cancer types and have been widely utilized for drug screening and molecular target identification. Screening these cell lines for envelope proteins or gene sequences related to xenotropic murine leukemia viruses (X-MLVs) revealed that one cell line, EKVX, was a candidate for production of an infectious gammaretrovirus. The presence of a retrovirus infectious to human cells was confirmed by the cell-free transmission of infection to the human prostate cancer cell line LNCaP. Amplification and sequencing of additional proviral sequences from EKVX confirmed a high degree of similarity to X-MLV. The cell line EKVX was established following passage of the original tumor cells through nude mice, providing a possible source of the X-MLV found in the EKVX cells.
Keywords: EKVX; NCI-60; human cell line; xenotropic murine leukemia virus; gammaretrovirus