Tuesday, June 28, 2011

XMRV cannot infect NIH3T3 mouse cells as Drs Mendoza, Vaughan, and Dusty Miller claimed

by XMRV Global Advocacy on Monday, June 27, 2011:

The following was posted on mecfsforum.com by Gerwyn:

The paper below, from Ramon Mendoza, Andrew E. Vaughan, and A. Dusty Miller, made the following claim:

"The left half of XMRV is present in an endogenous retrovirus of NIH/3T3 Swiss mouse cells"

Here we used XMRV-specific PCR to search for a more closely related source of XMRV in mice. While we could not find a complete copy, we did find a 3,600 bp region of XMRV in an endogenous retrovirus present in NIH/3T3 cells. These results show that XMRV has clear ancestors in mice, and highlight another possible source of contamination in PCR assays for XMRV.


However, the following paper demonstrates that NIH/3T3 cells cannot be infected by XMRV.

"Common Inbred Strains of the Laboratory Mouse That Are Susceptible to Infection by Mouse Xenotropic Gammaretroviruses and the Human-Derived Retrovirus XMRV"

In contrast, the XPR1 receptor gene originally cloned from NIH 3T3 cells is not permissive for X-MLV entry (2, 42, 50).


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